Services |
Harvard and HSCI |
Non-Harvard Academic & Non-Profit |
For-Profit & Industry |
Economical Injection of ES cells: Investigator provides an aliquot of ES cells in suspension, which will be injected into 30 blastocysts. Injected embryos will be implanted into 3 recipients, and chimeric offspring will be transferred at weaning to the investigator's colony. |
$ 2,643 |
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Standard blastocyst injection: 1 ES cell clone injection into 30 blastocysts and transfer into 3 recipient foster mothers; including the expansion and preparation of ES cells for injection |
$ 3,606 |
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Discount package for injection of 2 ES cell clones: 2 ES cell clones will be injected into 30 blastocysts each and transferred into 6 recipients; including the ES cell culture, expansion and preparation for injection |
$ 6,014 |
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New Option for Blastocyst Injection: Quality control of 3 targeted ES cell clones by chromosome analysis, and subsequent injection of 1 clone that has at least 70% euploid cells. including the ES cell culture, expansion and preparation for injection |
$ 5,987 |
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Injection of ES clones from the KOMP/EUCOMM Repositories: Typically ES cell clones from KOMP/EUCOMM are generated on the inbred C57BL/6N cell lines, JM8A3N1, JM8A1N3 or JM8N4. Clones from these cell lines will be injected into blastocysts from the albino-B6. Including the cell culture, injection of 2 clones into 30 blastocysts each followed by transfer into 6 recipients |
$ 6,014 |
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Chromosome Analysis (per sample) |
TBD |
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Chimera Breeding and Germ Line Transmission Testing: Each chimera is setup with 2 females or 1 male per cage. Animals will be monitored daily, offspring will be weaned and transferred to investigator’s colony. Animal procurement for mating, per diems, tail biopsies, and genotyping are not included. |
$ 130 (per mating cage) |
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Per diems for mating cages and offspring after weaning (per cage, per day) |
$ 2.29 |
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To get started, please fill out the Service Request Form, and submit by email GMFmail@fas.harvard.edu, or fax to (617) 384-5008.
Terms of service:
- Expansion of 2 ES cell clones and preparation for injection.
- Injection of 30 blastocysts per clone, transfer of emrbyos into recipients.
- Monitor chimeras, report to investigator, separate offspring at weaning, transfer chimeras to investigator colony.
Not included in service:
- Pathogen screening: investigators which plan to have ES cells imported into the GMF are responsible to provide a current mycoplasma test with the cell samples. The OAR SOP gives detailed instructions.
- ES cell quality control: on special request we can provide chromosome counting of ES cell to assess the percentage of normal diploid cells.
Disclaimer:
The GMF does not make guarantees that ES cell clones will produce germ line transmitting chimeras.
Non-Harvard Academic & Non-Profit users pay 15% more than Harvard users. Service fees for Industry & For-Profit users are given upon request.
The GMF further reserves the right to change fees without prior notice.
(Return to Services and Fees listing)