Blastocyst Injection - including KOMP/EUCOMM ES cell clones

Services

Harvard and HSCI

Non-Harvard Academic  & Non-Profit

For-Profit & Industry

Economical Injection of ES cells:

Investigator provides an aliquot of ES cells in suspension, which will be injected into 30 blastocysts. Injected embryos will be implanted into 3 recipients, and chimeric offspring will be transferred at weaning to the investigator's colony.  

$ 1,980

 

 

Standard blastocyst injection:  

1 ES cell clone injection into 30 blastocysts and transfer into 3 recipient foster mothers; including the expansion and preparation of ES cells for injection

$ 2,650

 

 

Discount package for injection of 2 ES cell clones:

2 ES cell clones will be injected into 30 blastocysts each and transferred into 6 recipients; including the culture, expansion and preparation of ES cell clones for injection

$ 4,800

 

 

New Option for Blastocyst Injection:

Quality control of 3 targeted ES cell clones by chromosome counting, and subsequent injection of 1 clone that has at least 70% euploid cells.

$ 3,700

 

 

Injection of ES clones from the KOMP/EUCOMM Repositories:

Typically ES cell clones from KOMP/EUCOMM are generated on the inbred C57BL/6N cell lines, JM8A3N1, JM8A1N3 or JM8N4. Clones from these cell lines will be injected into blastocysts from the albino-B6.

Including the cell culture, injection of 2 clones into 30 blastocysts each followed by transfer into 6 recipients

$ 4,800

 

 

Chromosome Counting (per sample)

$ 350

 

 

Chimera Breeding and Germ Line Transmission Testing:

Each chimera is setup with 2 females. Animals will be monitored daily, offspring will be weaned and transferred to investigator’s colony. Purchase mice for mating, per diems, tail biopsies, and genotyping are not included.

$ 100

(per mating cage)

 

 

Per diems for mating cages and offspring after weaning (per cage, per day)

$ 1.80

 

 

To get started, please fill out the Service Request Form, and submit by email GMFmail@fas.harvard.edu, or fax to (617) 384-5008.

Terms of service:

  • Expansion of 2 ES cell clones and preparation for injection.
  • Injection of 30 blastocysts per clone, transfer of emrbyos into recipients. 
  • Monitor chimeras, report to investigator, separate offspring at weaning, transfer chimeras to investigator colony. 

Not included in service:

  • Pathogen screening: investigators which plan to have ES cells imported into the GMF are responsible to provide a current mycoplasma test with the cell samples. The OAR  SOP gives detailed instructions.
  • ES cell quality control: on special request we can provide chromosome counting of ES cell to assess the percentage of normal diploid cells.

Disclaimer:
The GMF does not make guarantees that ES cell clones will produce germ line transmitting chimeras.
Non-Harvard Academic & Non-Profit users pay 10% more than Harvard users. Service fees for Industry & For-Profit users are given upon request.
The GMF further reserves the right to change fees without prior notice.

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