Cryopreservation

Services

Harvard and HSCI

Non-Harvard Academic  & Non-Profit

For-Profit & Industry

Sperm Cryopreservation, including quality control (QC) test to 2-cell stage

$980

 

 

Recovery of life pups following sperm cryopreservation and QC

$495

 

 

Embryo Cryopreservation: Standard

  • Cryopreserve 200-300 embryos, in 10 straws
  • Including QC testing to generate life pups

$2,550

 

 

Embryo Cryopreservation: from IVF (Speed Cryo)

$2,660

 

 

Embryos and Sperm Cryopreservation: Economical Solution for Freezing

$1,250

 

 

Cryostorage Fee (per year)

Fee will be prorated at the time of completion

$50

 

 

PRIA panel PCR testing from Charles River Labs

$225

 

 

To initiate a Cryopreservation Job please fill out the Service Request Form, and  forward the signed form to the GMF office at GMFmail@fas.harvard.edu, as an email attachment or fax to (617) 384-5008.

Economical Solution for Freezing Embryos and Sperm:

GMF will freeze embryos from one mating experiment of 5 female mice, age 3 to 20 weeks, and 5 males, age 6 to 20 weeks, to produce approximately 50 to 100 embryos which will be frozen in 2 to 4 straws. Epidydimal sperm from one male will be frozen in 5 vials.

Standard Embryo Cryopreservation:

Be sure to have 5 fertile males, age 6 to 20 weeks, and at least 10 females, age 3 to 8 weeks from the desired strain. We will produce approximately 250 embryos by natural mating, freeze 2-cell embryos in up to 12 straws by control rate freezing, test thaw 2 straws, implant into pseudopregnant recipients to produce live offspring. Straws can be stored in the GMF cryostorage tank, which is secured and alarmed, for a fee of $40.00 per year.

Cryopreservation of Embryos produced by IVF (SpeedCryo):

We require at least two males, age 6 to 20 weeks, experienced. Arrange with OAR for transfer of the males to the GMF colony. We will then produce approximately 250 embryos by IVF using fresh sperm, freeze 2-cell embryos in up to 12 straws by control rate freezing, test-thaw 2 straws, implant into pseudopregnant recipients to produce live offspring. Straws will be stored in GMF cryostorage tank.

Sperm Cryopreservation:

We require two males, age 6 to 20 weeks, with successful mating experience. Arrange with OAR to transfer male mice to GMF colony. Epididymal sperm will be frozen in 10 vials or straws, which can be stored in the GMF liquid nitrogen-storage tank for a yearly fee of $40.00.

To initiate transfer of animals into the GMF colony for embryo production or for the collection of sperm, please download the Animal Transfer Request Form, fill in required information and submit to the Office of Animal Resources (OAR) for processing by sending a fax to (617) 495-4218.

Non-Harvard Academic & Non-Profit users pay 10% more than Harvard users. Service fees for Industry & For-Profit users are given upon request.

Disclaimer: The GMF reserves the right to change fees without prior notice.

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