Blastocyst Injection - including KOMP/EUCOMM ES cell clones

Services

Harvard - Internal (with 33-digit code)
Harvard - External
Non-Harvard Academic  & Non-Profit
For-Profit & Industry

Economical Injection of ES cells:

Investigator provides an aliquot of ES cells in suspension, which will be injected into 30 blastocysts. Injected embryos will be implanted into 3 recipients, and chimeric offspring will be transferred at weaning to the investigator's colony.  

$ 2,775
 
 
 

Standard blastocyst injection:  

1 ES cell clone injection into 30 blastocysts and transfer into 3 recipient foster mothers; including the expansion and preparation of ES cells for injection

$ 3,786
 
 
 

Discount package for injection of 2 ES cell clones:

2 ES cell clones will be injected into 30 blastocysts each and transferred into 6 recipients; including the ES cell culture, expansion and preparation for injection

$ 6,314
 
 
 

New Option for Blastocyst Injection:

Quality control of 3 targeted ES cell clones by chromosome analysis, and subsequent injection of 1 clone that has at least 70% euploid cells. including the ES cell culture, expansion and preparation for injection

$ 6,286
 
 
 

Injection of ES clones from the KOMP/EUCOMM Repositories:

Typically ES cell clones from KOMP/EUCOMM are generated on the inbred C57BL/6N cell lines, JM8A3N1, JM8A1N3 or JM8N4. Clones from these cell lines will be injected into blastocysts from the albino-B6.

Including the cell culture, injection of 2 clones into 30 blastocysts each followed by transfer into 6 recipients

$ 6,314
 
 
 
Chromosome Analysis (per sample)
TBD
 
 
 

Chimera Breeding and Germ Line Transmission Testing: 

Each chimera is setup with 2 females or 1 male per cage. Animals will be monitored daily, offspring will be weaned and transferred to investigator’s colony. Animal procurement for mating, per diems, tail biopsies, and genotyping are not included.

$ 136

(per mating cage)

 
 
 
Per diems for mating cages and offspring after weaning (per cage, per day)
$ 2.41
 
 
 

 

To get started, please fill out the Service Request Form, and submit the signed and dated form by email GMFmail@fas.harvard.edu as an attachment file.

Terms of service:

  • Expansion of 2 ES cell clones and preparation for injection.
  • Injection of 30 blastocysts per clone, transfer of embryos into recipients.
  • Monitor chimeras, report to investigator, separate offspring at weaning, transfer chimeras to investigator colony. 

Not included in service:

  • Pathogen screening: investigators which plan to have ES cells imported into the GMF are responsible to provide a current mycoplasma test with the cell samples. The OAR  SOP gives detailed instructions.
  • ES cell quality control: on special request we can provide chromosome counting of ES cell to assess the percentage of normal diploid cells.

Disclaimer:
The GMF does not make guarantees that ES cell clones will produce germ line transmitting chimeras.

Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code).

Non-Harvard Academic & Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry & For-Profit users are given upon request.


The GMF further reserves the right to change fees without prior notice.

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