# Blastocyst Injection - including KOMP/EUCOMM ES cell clones Sort**Services** Harvard - Internal (with 33-digit code) Harvard - External Non-Harvard Academic & Non-Profit For-Profit & Industry **Economical Injection of ES cells:** Investigator provides an aliquot of ES cells in suspension, which will be injected into 30 blastocysts. Injected embryos will be implanted into 3 recipients, and chimeric offspring will be transferred at weaning to the investigator's colony. $ 2,775 **Standard blastocyst injection:** 1 ES cell clone injection into 30 blastocysts and transfer into 3 recipient foster mothers; including the expansion and preparation of ES cells for injection $ 3,786 **Discount package for injection of 2 ES cell clones:** 2 ES cell clones will be injected into 30 blastocysts each and transferred into 6 recipients; including the ES cell culture, expansion and preparation for injection $ 6,314 **New Option for Blastocyst Injection:** Quality control of 3 targeted ES cell clones by chromosome analysis, and subsequent injection of 1 clone that has at least 70% euploid cells. including the ES cell culture, expansion and preparation for injection $ 6,286 **Injection of ES clones from the KOMP/EUCOMM Repositories:** Typically ES cell clones from KOMP/EUCOMM are generated on the inbred C57BL/6N cell lines, JM8A3N1, JM8A1N3 or JM8N4. Clones from these cell lines will be injected into blastocysts from the albino-B6. Including the cell culture, injection of 2 clones into 30 blastocysts each followed by transfer into 6 recipients $ 6,314 **Chromosome Analysis (per sample)** TBD **Chimera Breeding and Germ Line Transmission Testing:** Each chimera is setup with 2 females or 1 male per cage. Animals will be monitored daily, offspring will be weaned and transferred to investigator’s colony. Animal procurement for mating, per diems, tail biopsies, and genotyping are not included. $ 136 (per mating cage) **Per diems** for mating cages and offspring after weaning (per cage, per day) $ 2.41 To get started, please fill out the [Service Request Form](/file_url/113), and submit the signed and dated form by email as an attachment file. Terms of service: - Expansion of 2 ES cell clones and preparation for injection. - Injection of 30 blastocysts per clone, transfer of embryos into recipients. - Monitor chimeras, report to investigator, separate offspring at weaning, transfer chimeras to investigator colony. Not included in service: - Pathogen screening: investigators which plan to have ES cells imported into the GMF are responsible to provide a current mycoplasma test with the cell samples. The OAR [ SOP](/file_url/98) gives detailed instructions. - ES cell quality control: on special request we can provide chromosome counting of ES cell to assess the percentage of normal diploid cells. ***Disclaimer:*** The GMF does not make guarantees that ES cell clones will produce germ line transmitting chimeras. Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code). Non-Harvard Academic & Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry & For-Profit users are given upon request. *The GMF further reserves the right to change fees without prior notice.* (Return to [Services and Fees](/pages/services-and-fees) listing)