Cryopreservation

Services

Harvard and HSCI

Non-Harvard Academic  & Non-Profit

For-Profit & Industry

Mouse Sperm Cryopreservation, including cryo-sperm quality test to 2-cell embryo stage. Not including per diem.

$1,124

 

 

Recovery of live pups following sperm cryopreservation and cryo-sperm test. Not including per diem.

$676

 

 

Mouse Embryo Cryopreservation: Standard

  • Cryopreserve 250-300 embryos in straws.
  • Including cryo-embryos testing to generate live pups. Animal procurement and per diem will be charged extra.

$2,924

 

 

Embryo Cryopreservation: from IVF (Speed Cryo)

$4,155

 

 

Sperm and Embryo Cryopreservation: Economical

  • Cryopreserve sperm, including cryo-sperm quality test to 2 cell stage.
  • Cryopreserve 120-150 embryos in straws. Not including cryo-embryo testing.  Animal procurement and per diem will be charged extra.

$3,124

 

 

Cryostorage Fee (per year)

Fee will be prorated at the time of completion

$104

 

 

PRIA panel: PCR testing by Charles River Labs

TBD

 

 

 

To initiate a Cryopreservation Job please fill out the Service Request Form, and forward the signed and dated form to the GMF office at GMFmail@fas.harvard.edu, as an email attachment or fax to (617) 384-5008.

Economical Solution for Freezing Embryos and Sperm:

GMF will freeze embryos from mating experiment of 5-10 female mice, age 3 to 20 weeks, and 5-10 fertile males, age 8 to 20 weeks, to produce approximately 120 to 150 embryos which will be frozen in straws. Epidydimal sperm from two males will be frozen in 10 straws.
 

Standard Embryo Cryopreservation:

Be sure to have 5-10 fertile males, age 8 to 20 weeks, and at least 10-20 females, age 3 to 20 weeks from the desired strain. We will produce approximately 250 embryos by natural mating, freeze 2-cell embryos in up to 12 straws by control rate freezing, test thaw, and implant into pseudopregnant recipients to produce live offspring. Straws can be stored in the GMF cryostorage tank, which is secured and alarmed, for an annual fee.


Cryopreservation of Embryos produced by IVF (SpeedCryo):

We require at least two fertile males, age 8 to 20 weeks. We will then produce approximately 250 embryos by IVF using fresh sperm, freeze 2-cell embryos in up to 12 straws by control rate freezing, test thaw, and implant into pseudopregnant recipients to produce live offspring. Straws can be stored in GMF cryostorage tank, which is secured and alarmed, for an annual fee.


Sperm Cryopreservation:

We require two fertile males, age 8 to 20 weeks, with successful mating experience.  Epididymal sperm will be frozen in 10 straws, which can be stored in the GMF liquid nitrogen-storage tank for an annual fee.

Transferring animals into the Harvard managed animal facility will be initiated after the Service Request Form has been submitted. Fees relating to the transport of live mice to Harvard and any health testing required prior to importation are the responsiblilty of the exporting lab.

Non-Harvard Academic & Non-Profit users pay 15% more than Harvard users. Service fees for Industry & For-Profit users are given upon request.

Disclaimer: The GMF reserves the right to change fees without prior notice.

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