Embryonic Stem Cell Gene Targeting

 

Services

Harvard - Internal (with 33-digit code)

 

Harvard - External
Non-Harvard Academic  & Non-Profit
For-Profit & Industry

ES cell gene targeting service includes: 

  • Electroporation of ES cells, drug-selection, colony picking, expansion of clones and production of replica plates for genotyping by investigator
  • ES cell lines available: V6.5 (129 x B6 F1 hybrid), JM8A3 or JM8A3N1 (B6N from KOMP/EUCOMM)  
  • We provide 2 x 96 clones for genotyping.
$4,753
 
 
 
Optional: Extraction of DNA in 2 replica 96-well plates for genotyping (please indicate on Service Request Form)
$710
 
 
 
Optional: Expansion of replica ES clones in eight 24-well plates
$1,285
 
 
 
Expansion of targeted ES clones (per clone, 6-well)
$152
 
 
 
Chromosome Analysis (per sample), including cell sample preparation
TBD
 
 
 
Mycoplasma testing (per sample) including cell culture and preparation
$264
 
 
 
Genotyping by PCR, services may be available upon request
TBD
 
 
 

 

To get started please fill out the Service Request Form, and submit the signed form to the GMF office at GMFmail@fas.harvard.edu, as an email attachment.

Disclaimer:
The GMF does not provide any guarantees that targeted ES cell clones will be produced and/or identified, nor does it guarantee that targeted ES cell clones will produce germ line transmitting chimeras. However, experience over the past years 100+ projects revealed a success rate of over 95%.

Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code).

Non-Harvard Academic & Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry & For-Profit users are given upon request.

The GMF reserves the right to change fees without prior notice.

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