# Embryonic Stem Cell Gene Targeting Sort **Services** Harvard - Internal (with 33-digit code) Harvard - External Non-Harvard Academic & Non-Profit For-Profit & Industry ES cell gene targeting service includes: - Electroporation of ES cells, drug-selection, colony picking, expansion of clones and production of replica plates for genotyping by investigator - ES cell lines available: V6.5 (129 x B6 F1 hybrid), JM8A3 or JM8A3N1 (B6N from KOMP/EUCOMM) - We provide 2 x 96 clones for genotyping. $4,753 Optional: Extraction of DNA in 2 replica 96-well plates for genotyping (please indicate on Service Request Form) $710 Optional: Expansion of replica ES clones in eight 24-well plates $1,285 Expansion of targeted ES clones (per clone, 6-well) $152 Chromosome Analysis (per sample), including cell sample preparation TBD Mycoplasma testing (per sample) including cell culture and preparation $264 Genotyping by PCR, services may be available upon request TBD To get started please fill out the [Service Request Form](/file_url/96), and submit the signed form to the GMF office at , as an email attachment. ***Disclaimer:*** The GMF does not provide any guarantees that targeted ES cell clones will be produced and/or identified, nor does it guarantee that targeted ES cell clones will produce germ line transmitting chimeras. However, experience over the past years 100+ projects revealed a success rate of over 95%. Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code). Non-Harvard Academic & Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry & For-Profit users are given upon request. *The GMF reserves the right to change fees without prior notice.* (Return to [Services and Fees](/pages/services-and-fees) listing)