Strain Rederivation
Services | Harvard - Internal (with 33-digit code) | Harvard - External | Non-Harvard Academic & Non-Profit | For-Profit & Industry |
Resuscitation of cryopreserved mouse embryos to generate mouse colony: Receiving and storing frozen embryos in LN2, thawing up to 2 straws, culturing embryos, perform up to 3 embryo transfers, housing offspring until weaning. Per diem costs will be charged extra. | $1,248 for up to 3 recipients $240 for each additional recipient | |||
Producing live offspring from cryopreserved or fresh sperm through IVF (1 litter): Per diem costs and mouse purchase for oocyte donors will be charged extra. | $2,242 for up to 3 recipients $240 for each additional recipient | |||
Generate live offspring from fresh embryos: Receiving fresh embryos, performing embryo transfers into recipients upon arrival. Housing offspring until weaning. Per diem costs will be charged extra. | $1,248 for up to 3 recipients $240 for each additional recipient | |||
Rederivation of mouse strain by embryo transfer: to generate pathogen free mouse lines. Per diem costs and mouse purchase for sperm or oocyte donors will be charged extra. | $1,321 for up to 3 recipients $240 for each additional recipient |
To initiate a Strain Rederivation Job please fill out the Service Request Form, and forward the signed form to the GMF office at GMFmail@fas.harvard.edu, as an email attachment.
Investigator Responsibilities:
- For cryopreserved embryos: arrange for shipment of at least 2 straws of embryos from the supplier to the GMF laboratory in dry LN2 shipping container. (Investigator carries the costs for shipment of the dry LN2 shipping container).
- Receiving live embryos at ambient temperature: arrange with supplier for availability of strain and approximate timeframe. GMF will schedule the time of shipping.
- For cryopreserved sperm: arrange for shipment of at least 2 straws/vials of sperm from the supplier to the GMF laboratory in dry LN2 shipping container. (Investigator carries the costs for shipment of the dry LN2 shipping container.)
- All rederivations are subject to veterinary review, approval, and health testing at investigator cost. Please solicit health report from suppling institution prior to sample shipment.
GMF Responsibilities:
- Schedule shipping date of sample with sender or investigator.
- Receive sample in and store securely until rederivation procedure is performed.
- Perform rederivation procedure based on sample type. Embryo transfers will be into clean pseudopregnant recipient mice.
- Transfer recipient animals to quarantine area for temporary holding during gestation and until weaning. Assist in coordinating any serological testing needed with FAS-OAR.
Disclaimer:
Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code).
Non-Harvard Academic & Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry & For-Profit users are given upon request.
The GMF reserves the right to change fees without prior notice.
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