#  DNA Microinjection 

 



Sort**Services**



Harvard - Internal (with 33-digit code)

Harvard - External

Non-Harvard Academic & Non-Profit

For-Profit & Industry

Plasmid transgenes injection in F1-hybrid (BDF1) embryos. Not including male colony

 $ 3,976

 

 

 

BAC or YAC transgenes injection in F1-hybrid (BDF1) embryos. Not including male colony

 $ 4,732

 

 

 

Plasmid transgenes injection in inbred (C57BL/6) embryos

 $ 5,050

 

 

 

BAC or YAC transgene injection in inbred (C57BL/6) embryos

 $ 6,232

 

 

 

Per diems for recipient cages and offspring after weaning (per cage, per day)

 $ 2.41

 

 

 

Tail biopsy (per sample)

 $ 9.99

 

 

 

Transgene DNA purification by electroelution and purification for microinjection (per prep)

 $ 937

 

 

 

Plasmid transgenes injection in embryos of mutant or other wild type strain

TBD

 

 

 

BAC or YAC transgenes injection in mutant embryos

TBD

 

 







To get started please fill out the [Service Request Form](/file_url/96) and submit the signed and dated form by email to <GMFmail@fas.harvard.edu> as an attachment file.

Terms of Service:

- Microinjection of approximately 200 embryos with transgene DNA, transfer of embryos into surrogate recipient animals, weaning offspring, and transferring transgenic offspring to investigator colony.
- We strive to provide at least 3 transgenic founder animals. If this number cannot be generated during the standard service, we will provide a repeat injection with fee.
- Investigators are requested to provide details of the purified transgene by submitting the [sample sheet](/file_url/102) together with the DNA.
- Investigators who do not have prior experience with transgene purifications may make a request for the GMF to provide that service. Investigators with experience are encouraged to use our standard [DNA purification](/file_url/97) protocol.

***Disclaimer:***

*The GMF does not make any guarantees for specific numbers of founders, but the success rate over the past years has exceeded 98% for some 450 projects, and the transgenic frequency has routinely been above 15%.*

Harvard-External users pay 8% more than Harvard-Internal users (with 33-digit code).

Non-Harvard Academic &amp; Non-Profit users pay 18% more than Harvard-Internal users. Service fees for Industry &amp; For-Profit users are given upon request.

*The GMF further reserves the rights to change the fee schedule without special notice to adjust for special financial circumstances.*

(Return to [Services and Fees](/pages/services-and-fees) listing)



 

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 Attachments- [  picture\_as\_pdf  gmf\_service\_request\_form.pdf ](/sites/g/files/omnuum9991/files/genome-modification-facility/files/gmf_service_request_form.pdf)
- [  picture\_as\_pdf  transgene\_dna\_purification\_2017.pdf ](/sites/g/files/omnuum9991/files/genome-modification-facility/files/transgene_dna_purification_2017.pdf)
- [  picture\_as\_pdf  transgene\_dna\_sample\_sheet.pdf ](/sites/g/files/omnuum9991/files/genome-modification-facility/files/transgene_dna_sample_sheet.pdf)
 
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